rabbit polyclonal anti pink1  (Novus Biologicals)

Journal: bioRxiv

Article Title: Mitophagosomes induced during EV-D68 infection promote viral nonlytic release

doi: 10.1101/2024.12.05.627125

Figure Lengend Snippet: (A) H1HeLa cells were transfected with the scramble and Parkin siRNAs for 48 h. The cells were then infected with EV-D68 for 5 h and viral titers were measured by a plaque assay. (B) H1HeLa cells were transfected with siRNAs against the mitophagy receptor, BCL2L13 for 48 h. The cells were then infected as in A for a plaque assay-based viral titer measurement. (C) A549 cells were transfected with scramble, Parkin, and PINK1 siRNAs for 48 h. The cells were infected with EV-D68 for 5h and viral titers were determined by a plaque assay. (D) A549 cells were transfected with the indicated siRNAs for 48 h. The cells were then infected and tittered as in A. (E) A549 cells were transfected with the indicated mitophagy-associated siRNAs for 48 h, followed by viral infection and titer determination as in A. An MOI of 0.1 was used for all infections. Error bars indicate mean ± SEM of at least 2 independent repeats. Unpaired student’s t-test was used for the statistical analyses (**= p< 0.01; *= p< 0.05; ns=not significant.).

Article Snippet: Rabbit polyclonal-anti-Pink1 (Novus Biologicals, BC100-494).

Techniques: Transfection, Infection, Plaque Assay

Journal: Clinical Science (London, England : 1979)

Article Title: Impaired cardiac non-neuronal acetylcholine synthesis triggers mitochondrial dysfunction with the loss of nicotinic receptor-mediated calcium handling, causing the failing heart

doi: 10.1042/CS20257026

Figure Lengend Snippet: A . LC3-II or LC3-I/II ratio was altered in hChAT KO mouse hearts, associated with the up-regulation of p62 and nitrotyrosine protein expression. B , Protein levels of both Parkin (PRK) and PINK1 were altered reciprocally between cytosolic and mitochondrial fractions of hChAT KO mouse hearts, compared with control mouse hearts. The hChAT KO reduced PRK and PINK1 protein levels in the cytosolic fraction ( P <0.05 and P <0.05, respectively), however alternatively, their levels both increased in the mitochondrial fractions ( P <0.01 and P <0.01, respectively).

Article Snippet: Furthermore, proteins from mitochondrial or cytosol fraction were subjected to western blot analysis using a rabbit anti-PARK2/Parkin polyclonal antibody (1:1000, #14060–1-AP; Proteintech), rabbit anti-PINK1 polyclonal antibody (1:1000, #23274–1-AP; Proteintech), rabbit anti-GAPDH monoclonal ab (Cell Signaling Technology), and rabbit anti-VDAC polyclonal ab (Abcam).

Techniques: Expressing, Control

Journal: The FASEB Journal

Article Title: Tmx2 Maintains Mitochondrial Function to Support Preimplantation Embryogenesis

doi: 10.1096/fj.202500640r

Figure Lengend Snippet: FIGURE 6 | Tmx2 knockdown inhibits mitophagy and autophagy in morula-stage embryos. (A-D) Immunostaining for PINK1 (A), PARKIN (B), MAP1LC3B (C), and LAMP1 (D) in control and Tmx2-knockdown embryos. Scale bars: 75 μm. (E-H) Quantification of immunofluorescence inten- sity for PINK1 (E), PARKIN (F), MAP1LC3B (G), and LAMP1 (H) in control and Tmx2-knockdown morula-stage embryos. Sample sizes: PINK1: Control = 16, siRNA2 = 14, siRNA3 = 19; PARKIN: Control = 21, siRNA2 = 20, siRNA3 = 23; MAP1LC3B: Control = 22, siRNA2 = 20, siRNA3 = 21; LAMP1: Control = 30, siRNA2 = 32, siRNA3 = 31. Statistical significance: **p < 0.01, ***p < 0.001 and ****p < 0.0001.

Article Snippet: The primary antibodies used included: rabbit anti- TMX2 (Origene, TA341391, 1:50), goat anti- OCT4 (Abcam, ab27985, 1:200), mouse antiCDX2 (Biogenex, MU392A- UC, 1:100), goat anti- SOX17 (R&D Systems, AF1924, 1:100), rabbit anti- NANOG (Abcam, ab80892, 1:50), rabbit anti- PINK1 (Affinity, DF7742, 1:50), rabbit antiPARKIN (Abcepta, AP6402B, 1:50), rabbit anti- MAP1LC3B (CUSABIO, CSB- PA013403GA01HU, 1:50), and rabbit antiLAMP1 (Beyotime, AF7353, 1:100).

Techniques: Knockdown, Immunostaining, Control, Immunofluorescence

Journal: Biology

Article Title: Consequences of Early Maternal Deprivation on Neuroinflammation and Mitochondrial Dynamics in the Central Nervous System of Male and Female Rats

doi: 10.3390/biology13121011

Figure Lengend Snippet: Antibodies used in the present study.

Article Snippet: Rabbit anti-PINK1 polyclonal (63 kDa) , Thermo Fisher (PA1-16604) , 1:1000 , , , .

Techniques: